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KMID : 0391319960060010045
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Korean Journal of Biological Response Modifiers
1996 Volume.6 No. 1 p.45 ~ p.56
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Autografting of Peripheral Blood Progenitor Cells Mobilized by Combination of Chemotherapy and Hematopoietic Growth Factors for the Treatment of Advanced Cancer
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Choi Kyeoung-Sang
Kim Hyeoung-Joon
Kook Hoon
Chung Ik-Joo
Park Moo-Rim
Seo Jae-Sung
Hwang Tai-Ju
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Abstract
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Background: Peripheral blood stem cell transplantation(PBSCT) is a relatively new therapeutic modality in the treatment of various malignancies. To obtain enough peripheral blood stem cells (PBSC) ensuring transplantation, collection of PBSC by a series of leukaphereses is needed after mobilization of PBSC from bone marrow. The most effective method of mobilization and the best timing of leukapheresis have yet to be clarified.
Methods: PBSC were collected by 54 leukaphereses in 13 patients with various malignancies after combined mobilization of chemotherapy and granulocyte or granulocyte-macrophage colony stimulating factors.
Results:
1) Peripheral blood leukocyte count and CD34+ cell number on the day of leukapheresis had significant correlation with both colony forming unit-granulocyte macrophage(CFU-GM)/kg and CD34+ /kg numbers after collection, implicating good predictors of timing of leukapheresis.
2) The number of peripheral blood CFU-GM was increased 3 times after mobilization even in patients who received intensive chemotherapy previously.
3) Progenitor yield of leukapheresis was not affected by repeated mobilization cycles.
4) The high rate of coexpression of CD33+, CD38+, and HLA-DR+ in mobilized peripheral CD34+ cells suggested that most CD34+ cells after mobilization represented committed progenitor cells facilitating short-term hematopoiesis.
5) In 6 patients who underwent PBSCTs, early engraftment of neutrophil and platelets was documented. Long-term engraftment has also been demonstrated in 3 patients living up to 420 days.
Conclusion: The combination of chemotherapy and growth factors seemed to be effective method of PBSC mobilization to ensure a successful PBSCT in the treatment of various malignancies.
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KEYWORD
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Peripheral blood stem cell transplantation, Perpheral blood stem cell, Colony stimulating factors, CD34, Mobilization
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